22RV1-CAS9 cell line

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22RV1-CAS9 Order

Catalog#

YC-C012-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-B

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

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Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

22RV1-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:3

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90% RPMI-1640+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the 22RV1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a 22RV1-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
22Rv1-CAS9	Cas9	16.07987213	1.84493923
22Rv1-CAS9	β-actin	14.23493290	1.84493923
22RV1	Cas9	30.99718666	14.82471085
22RV1	β-actin	16.17247581	14.82471085

Cutting Efficiency Validation

22RV1-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the 22Rv1-CAS9 stable cell pool which is electroporated by AR gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:22RV1			STR Info (Cell bank) Cell Line:22RV1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11	12	13.0
D13S317	9	12		9	12
D7S820	9	10	11.0	10	11	9.0
D16S539	12	12		12	12
VWA	15	21		15	21
TH01	6	9.3		6	9.3
AMEL	X	Y		X	Y
TPOX	8	8		8	8
CSF1PO	10	11		10	11
D12S391	18	25
FGA	20	23
D2S1338	17	18
D21S11	29	30
D18S51	13	14
D8S1179	13	14
D3S1358	14	15
D6S1043	16	16
PENTAE	5	13	14.0
D19S433	13	14
PENTAD	9	12
D1S1656	17	18	19.0

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

22RV1-CAS9

Catalog#

YC-C012-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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22RV1-CAS9 cell line

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22RV1-CAS9 Order

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Product Advantage

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